Abstract
Light scattering properties of bacterial cells m ostly depend on their sizes, refractive indexes of their com ponents and surrounding environment. Interaction between bacterial cells and 3d3 type transition m etals causes their optical characteristics’ changes. Desulfuromonas acetoxidans are uncolored gram -negative obligatory anaerobic sulfur reducing bacteria that can be used as m icrobial fuel cells with high electron recovery from different organic com pounds oxidation to electric current as a result of electrons transfer in the processes of sulfur and som e 3d3 type transition m etals reduction, such as Ferrum and M anganese. In this study size distribution and relative content in the chosen interval of sizes (0.2 –2.0 mum ) of sulfur reducing D. acetoxidans bacterial cells under the influence of different concentrations of m anganese chloride (II) hexahydrate, ferrous chloride (III) hexahydrate and ferrous sulfate (II) have been investigated by the new m ethod of m easuring. A m ethod includes sounding of flow suspended bacterial cells by m onochrom atic coherent light, registration of signals of co-operation of sounding radiation with the explored m icrobiological objects by detects am plitudes and durations of scattered light im pulses. Correlation between changes of light-scattering properties and growth of Desulfuromonas acetoxidans cells under these conditions has been shown.
© 2011 OSA/SPIE
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