Calibration phantom for the quantification of fluorescent labels in deep skin tissue

Sebastian Beer; Ulf Maeder; Thorsten Bergmann; Peggy Schlupp; Thomas Schmidts; Johannes T. Heverhagen; Frank Runkel; Martin Fiebich

doi:10.1364/ECBO.2011.80861L

Advanced Microscopy Techniques II
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2011),
paper 80861L
•https://doi.org/10.1364/ECBO.2011.80861L

Calibration phantom for the quantification of fluorescent labels in deep skin tissue

Sebastian Beer, Ulf Maeder, Thorsten Bergmann, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, and Martin Fiebich

European Conference on Biomedical Optics 2011

Munich Germany
22–26 May 2011
ISBN: 9780819486837

From the session
POSTER SESSION (PSI)

Not Accessible

Your library or personal account may give you access

Get PDF
Email
Share
Get Citation
Copy Citation Text
S. Beer, U. Maeder, T. Bergmann, P. Schlupp, T. Schmidts, J. T. Heverhagen, F. Runkel, and M. Fiebich, "Calibration phantom for the quantification of fluorescent labels in deep skin tissue," in Advanced Microscopy Techniques II, E. Beaurepaire and P. So, eds., Vol. 8086 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2011), paper 80861L.

Export Citation
- BibTex
- Endnote (RIS)
- HTML
- Plain Text
Citation alert
Save article

Abstract

A method to quantify fluorescent labels spatially resolved in scattering and absorbing samples is proposed and tested using a tissue phantom. The method works without any a priori knowledge about the optical properties of the sample. The scattering and absorption behavior of the sample is estimated by measuring reflectance from the sample simultaneously to the fluorescence. With this estimation, the attenuation of the fluorescence caused by scattering and absorption can be mathematically compensated. The method is planned to be used for evaluating skin penetrating drug carrier systems.

PDF Article

More Like This

4D confocal microscopy method for drug localization in the skin

Ulf Maeder, Thorsten Bergmann, Jan Michael Burg, Sebastian Beer, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, and Martin Fiebich
80861A European Conference on Biomedical Optics (ECBO) 2011

Label-free Quantification of Pharmacokinetics in Skin

Amin Feizpour, Louise Bastholm, Troels T. Marstrand, and Conor L. Evans
JW3C.1 Bio-Optics: Design and Application (BODA) 2019

Quantification of Fluorescence Target in Tissue Phantoms by Time-domain Diffuse Optical Tomography with Phantoms – Total-light Approach

Goro Nishimura, Kamlesh Awasthi, Kitsakorn Locharoenrat, Shinpei Okawa, and Yukio Yamada
BTuD11 Biomedical Optics (BIOMED) 2010

Previous Article Next Article