Point spread function measured in human skin using two-photon fluorescence microscopy

Stina Guldbrand; Carl Simonsson; Maria Smedh; Marica B. Ericson

doi:10.1364/ECBO.2009.7367_1R

Advanced Microscopy Techniques
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2009),
paper 7367_1R
•https://doi.org/10.1364/ECBO.2009.7367_1R

Point spread function measured in human skin using two-photon fluorescence microscopy

Stina Guldbrand, Carl Simonsson, Maria Smedh, and Marica B. Ericson

European Conference on Biomedical Optics 2009

Munich Germany
14–18 June 2009
ISBN: 9780819476432

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S. Guldbrand, C. Simonsson, M. Smedh, and M. B. Ericson, "Point spread function measured in human skin using two-photon fluorescence microscopy," in Advanced Microscopy Techniques, P. Campagnola, E. Stelzer, and G. Von Bally, eds., Vol. 7367 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2009), paper 7367_1R.

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Abstract

The two-photon excitation point spread function (TPE-PSF) has been measured in human skin in vitro in order to examine the optical resolution. This has been done by injecting fluorescent subresolution beads in skin samples using a syringe. The beads were imaged at different depths and the full width at half maximum (FWHM) of the TPE-PSF in the lateral and axial direction were measured from the intensity profile of the emission. The experimentally obtained values of the PSF widths were larger than calculated values. Both the lateral FWHM and the axial FWHM were broadened as a function of depth but the increase was stronger in the axial direction. This indicates that the optical properties of the skin have a more pronounced effect of the resolution in the axial direction.

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