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Conferences > ECBO > 2009 > PSI > 7367_1E

Applying Image Restoration to Fluorescence Lifetime Imaging Microscopy (FLIM)

Ching-Wei Chang and Mary-Ann Mycek

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Abstract

We describe a novel approach using 2D-intensity-deconvolution to improve spatial resolution in wide-field FLIM. The method maintains lifetime accuracy and can restore features within experimentally reasonable intensity ranges.

© 2009 OSA/SPIE

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