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Chiral chromophores for second harmonic microscopy

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Abstract

The use of chiral harmonophores in second harmonic generation (SHG) microscopy of lipid bilayers should enable one to obtain a signal even when the distribution of the chromophores is centrosymmetric. In order to determine optimal chiral molecules, we performed polarization-resolved second harmonic reflection experiments. We found that chirality must arise from an excitonic coupling rather than from an asymmetric center. We selectively- labeled giant unilamellar lipid vesicles and cell membranes with such a molecule, namely an acridine substituted Troger’s base, as demonstrated by two-photon-excited fluorescence microscopy. We performed preliminary SHG microscopy experiments, but the poor efficiency of the current form of our molecule does not allow us to demonstrate chirality effects.

© 2003 SPIE

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