Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Second harmonic generation imaging in muscle fibers

Not Accessible

Your library or personal account may give you access

Abstract

We have used second harmonic generation (SHG) imaging to quantify a strong intrinsic SHG-signal from cellular and subcellular muscle fibre preparations. In isolated single muscle cells, the intrinsic SHG-signal periodically follows the striation pattern and strongly depends on the sarcomere length and the polarization of the illuminating laser beam. At the subcellular level, the SHG signal seems to be located mainly at the overlapping region of the (thin) actin and (thick) myosin filaments. Thus, SHG imaging resolves the arrangement of the contractile structures with high resolution non-invasively and without chromophores. It may also allow to study dynamic molecular interactions of the motor protein myosin with actin filaments during force production and muscle shortening.

© 2003 SPIE

PDF Article
More Like This
Functional imaging of skeletal muscle fiber in different physiological states by Second Harmonic Generation

V. Nucciotti, C. Stringari, L. Sacconi, F. Vanzi, C. Tesi, N. Piroddi, C. Poggesi, C. Castiglioni, A. Milani, M. Linari, G. Piazzesi, V. Lombardi, and F.S. Pavone
6630_3 European Conference on Biomedical Optics (ECBO) 2007

Real-Time Studies of Muscle Cell Contractions with Second Harmonic Generation Microscopy

N. Prent, R. Cisek, C. Greenhalgh, A. Major, B. Stewart, and V. Barzda
CWE3 Conference on Lasers and Electro-Optics (CLEO:S&I) 2009

Second Harmonic Generation Imaging Microscopy of Endogenous Structural Proteins

Paul J. Campagnola, Andrew Millard, and William A. Mohler
ThEE3 Frontiers in Optics (FiO) 2003

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved