Reconstruct-to-refocus: joint reconstruction of a biological sample and of calibration objects for accurate auto-focusing in digital holography

Dylan Brault; Anthony Berdeu; Anthony Berdeu; Ferréol Soulez; Fabien Momey; Thomas Olivier; Loïc Denis; Corinne Fournier

doi:10.1364/DH.2021.DW4C.8

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper DW4C.8
•https://doi.org/10.1364/DH.2021.DW4C.8

Reconstruct-to-refocus: joint reconstruction of a biological sample and of calibration objects for accurate auto-focusing in digital holography

Dylan Brault, Anthony Berdeu, Ferréol Soulez, Fabien Momey, Thomas Olivier, Loïc Denis, and Corinne Fournier

Digital Holography and Three-Dimensional Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Digital Holographic Microscopy II (DW4C)

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D. Brault, A. Berdeu, F. Soulez, F. Momey, T. Olivier, L. Denis, and C. Fournier, "Reconstruct-to-refocus: joint reconstruction of a biological sample and of calibration objects for accurate auto-focusing in digital holography," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper DW4C.8.

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Abstract

We propose to reconstruct biological samples using calibration beads to perform numerical autofocusing in in-line holographic microscopy. The approach is based on a joint inversion using both a parametric and a regularized inversion method.

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