Abstract

Conventional optical imaging technique are only sensitive to the light intensity. However, many other optical parameters can be probed to enhance the quantity of information retrieved from a biological sample. I will discuss in this presentation how and why measuring the so-called phase of the light. Applications both in the scope of label-free microscopy and for 3D fluorescence super-resolution will be discussed. In particular, I will show that this quantitative imaging modality allows to identify without labeling many organelles at high frame-rate and for any duration1 in living cells. Then I will move to the application of imaging both the intensity and the phase of fluorescent single emitter and demonstrate that it leads to single molecule 3D localization and 3D super-resolution even at depth in biological tissues2

© 2019 The Author(s)

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