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Autofocus in digital holography by the first order longitudinal difference

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Abstract

We propose an auto-focusing method in digital holography by computing the first order longitudinal difference among adjacent reconstruction planes. Our method is experimentally demonstrated for the refocusing of different kinds of objects. It is shown that our method is robust and faster than other representative methods. Our method may find applications in long-term investigations of living cells, where fast adjustment of numerical focus is required.

© 2016 Optical Society of America

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