Quantitative characterization of cellular adhesions with Total Internal Reflection Holographic Microscopy

William M. Ash; David Clark; Chun-Min Lo; Myung K. Kim

doi:10.1364/DH.2010.DTuA4

Biomedical Optics and 3-D Imaging
OSA Technical Digest (CD) (Optica Publishing Group, 2010),
paper DTuA4
•https://doi.org/10.1364/DH.2010.DTuA4

Quantitative characterization of cellular adhesions with Total Internal Reflection Holographic Microscopy

William M. Ash, David Clark, Chun-Min Lo, and Myung K. Kim

Digital Holography and Three-Dimensional Imaging 2010

Miami, Florida United States
12–14 April 2010
ISBN: 978-1-55752-887-2

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Holographic Microscopy (DTuA)

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W. M. Ash, D. Clark, C. Lo, and M. K. Kim, "Quantitative characterization of cellular adhesions with Total Internal Reflection Holographic Microscopy," in Biomedical Optics and 3-D Imaging, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper DTuA4.

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Abstract

Total Internal Reflection Holographic Microscopy (TIRHM) uses near-field phase shifts to quantitatively image cellular adhesions. Cell-substrate interfaces cause relative index of refraction and frustrated TIR to modulate the specimen’s phase profile. Dictyostelium Discoideum imagery presented.

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