Abstract

An experimental decoupling procedure that separates both the thickness and the relative refraction index of transparent microscopic objects is discussed. Digital Holography Microscopy (DHM) provides the separate reconstruction of the thickness and the relative refraction index of the sample under study. Such maps are obtained form the phase difference maps reconstructed from the digital holograms of the sample, immersed into different media. Experimental results for a glass capillary are presented.

© 2009 Optical Society of America