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Holographic imaging of pulsatile microvascular blood flow in the cerebral cortex

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Abstract

Imaging of microvascular blood flow in the exposed cerebral cortex of a mouse was achieved by laser holographic interferometry with a 50 kHz framerate camera. Videos of local Doppler contrasts were rendered numerically by Fresnel transformation and short-time Fourier transform analysis of off-axis holograms. Pulsatile blood flow contrasts in superficial blood vessels were revealed with a spatial resolution of 10 microns and a temporal resolution of 20 ms.

© 2016 Optical Society of America

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