Single-Shot Fluorescence Lifetime Imaging Microscopy Utilising a Time-Folding Optical Cavity

Guillem Carles; Valentin Kapitany; Vytautas Zickus; Daniele Faccio

doi:10.1364/COSI.2021.CTu4B.6

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper CTu4B.6
•https://doi.org/10.1364/COSI.2021.CTu4B.6

Single-Shot Fluorescence Lifetime Imaging Microscopy Utilising a Time-Folding Optical Cavity

Guillem Carles, Valentin Kapitany, Vytautas Zickus, and Daniele Faccio

Computational Optical Sensing and Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Computational Microscopy II (CTu4B)

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G. Carles, V. Kapitany, V. Zickus, and D. Faccio, "Single-Shot Fluorescence Lifetime Imaging Microscopy Utilising a Time-Folding Optical Cavity," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper CTu4B.6.

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Abstract

We report a new technique for fluorescence lifetime imaging microscopy, using a time-folded optical cavity and a gated camera, capable of retrieving arbitrarily-high-resolution lifetime maps from single-shot acquisitions. The technique enables lifetime imaging at video-rate.

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