Local methods to improve cryo-electron microcopy maps

S. Kaur; J. Gomez-Blanco; A. Khalifa; S. Adinarayanan; R. Sanchez-Garcia; R. Sanchez-Garcia; D. Wrapp; J. S. McLellan; K. H. Bui; J. Vargas

doi:10.1364/COSI.2021.CTu4B.3

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper CTu4B.3
•https://doi.org/10.1364/COSI.2021.CTu4B.3

Local methods to improve cryo-electron microcopy maps

S. Kaur, J. Gomez-Blanco, A. Khalifa, S. Adinarayanan, R. Sanchez-Garcia, D. Wrapp, J. S. McLellan, K. H. Bui, and J. Vargas

Computational Optical Sensing and Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Computational Microscopy II (CTu4B)

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S. Kaur, J. Gomez-Blanco, A. Khalifa, S. Adinarayanan, R. Sanchez-Garcia, D. Wrapp, J. S. McLellan, K. H. Bui, and J. Vargas, "Local methods to improve cryo-electron microcopy maps," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper CTu4B.3.

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Abstract

Cryo-electron microscopy (cryo-EM) has become a powerful technique for protein structure determination. However, there are still important issues to be addressed, such as, robust sharpening or local B-factors estimations. To address these issues, we present here new local methods to improve the analysis and interpretability of cryo-EM maps.

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