Extended-Range 3D Localization Microscopy using the COSA Phase Mask

Guillem Carles

doi:10.1364/COSI.2021.CM1A.2

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper CM1A.2
•https://doi.org/10.1364/COSI.2021.CM1A.2

Extended-Range 3D Localization Microscopy using the COSA Phase Mask

Guillem Carles

Computational Optical Sensing and Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Computational Microscopy I (CM1A)

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G. Carles, "Extended-Range 3D Localization Microscopy using the COSA Phase Mask," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper CM1A.2.

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Abstract

Precise 3D-localization of emitters over > 20× the depth-of-field of the microscope is possible using the COSA phase mask. Two localization algorithms that show a compromise between speed and precision are compared for different signal-to-noise levels.

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