How to assess depth-of-field extension strategies applied to single-molecule super-resolution microscopes?

Olivier Lévêque; Caroline Kulcsár; Antony Lee; Antony Lee; Pierre Bon; Pierre Bon; Laurent Cognet; Laurent Cognet; François Goudail

doi:10.1364/COSI.2021.CF4B.5

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper CF4B.5
•https://doi.org/10.1364/COSI.2021.CF4B.5

How to assess depth-of-field extension strategies applied to single-molecule super-resolution microscopes?

Olivier Lévêque, Caroline Kulcsár, Antony Lee, Pierre Bon, Laurent Cognet, and François Goudail

Computational Optical Sensing and Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Super-resolution (CF4B)

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O. Lévêque, C. Kulcsár, A. Lee, P. Bon, L. Cognet, and F. Goudail, "How to assess depth-of-field extension strategies applied to single-molecule super-resolution microscopes?," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper CF4B.5.

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Abstract

Phase masks used to extend the depth-of-field (DoF) of localization microscopes are often designed to increase localization accuracy despite a loss in detection probability. We propose a method to optimize DoF extended single-molecule localization techniques taking into account the trade-off between detection probability and localization accuracy.

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