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Conferences > COSI > 2019 > CW4A > CW4A.1

Techniques for methodical, optical and computational automation in light-sheet microscopy

Caroline Müllenbroich, Ludovico Silvestri, Lapo Turrini, Antonino Paolo Di Giovanna, Irene Costantini, Giacomo Mazzamuto, Francesco Vanzi, Leonardo Sacconi, Carl J. Nelson, Jonathan Taylor, and Francesco Saverio Pavone

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Abstract

Light-sheet microscopy excels in fast whole-organ acquisitions either in clarified mouse brains or intrinsically transparent zebrafish larva. Here, we present our technical and optical solutions for microscope automation including autofocusing, Bessel beams and prospective gating.

© 2019 The Author(s)

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