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3D Phase Retrieval with Computational Illumination

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Abstract

This talk will describe new methods for achieving 3D and high-resolution images in a commercial microscope with Fourier Ptychography related methods and a multi-slice approach. The setup involves coding on the illumination side by replacing the illumination unit of the microscope with a programmable LED array for computational illumination. Using a combination of optical hardware and post-processing software, we achieve real-time 3D and phase imaging with digital aberration correction. The result is a high-resolution gigapixel image in multiple depth planes with fast capture times. Such computational approaches to optical microscopy add significant new capabilities to commercial microscopes without significant hardware modification.

© 2015 Optical Society of America

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