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Optical resolution in wide-field oblique plane microscopy

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Abstract

Wide-field oblique plane microscopy (WOPM) enables direct, two-dimensional (2D) imaging of any inclined plane of interest in a three-dimensional (3D) object. It is suitable for real-time biological imaging of living samples whose structures change rapidly or develop in arbitrary orientations. WOPM has been implemented by a remote focusing technique with a tilted mirror, but its optical resolution has only been roughly estimated. Here, we formulate WOPM’s point spread function (PSF) using the vector diffraction theory to accurately predict the resolving power. We numerically demonstrate WOPM’s anisotropic lateral resolution from the calculated PSF.

© 2014 Optical Society of America

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