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  • Frontiers in Optics 2009/Laser Science XXV/Fall 2009 OSA Optics & Photonics Technical Digest
  • OSA Technical Digest (CD) (Optica Publishing Group, 2009),
  • paper CThA4
  • https://doi.org/10.1364/COSI.2009.CThA4

Localization Precision of Three-Dimensional Superresolution Fluorescence Imaging Using a Double-Helix Point Spread Function

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Abstract

We localize a diffraction-limited fluorescent bead to 10-20 nm in three dimensions using a double-helix point spread function and use this method to track a fluorescently tagged protein in three dimensions within a live cell.

© 2009 Optical Society of America

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