Super-resolution Deep Imaging with Gauss-Bessel STED Microscopy

Wentao Yu; Ziheng Ji; Xusan Yang; Yunfeng Xiao; Yunfeng Xiao; Peng Xi; Kebin Shi; Kebin Shi

doi:10.1364/CLEO_SI.2016.STh4G.4

Conference on Lasers and Electro-Optics
OSA Technical Digest (2016) (Optica Publishing Group, 2016),
paper STh4G.4
•https://doi.org/10.1364/CLEO_SI.2016.STh4G.4

Super-resolution Deep Imaging with Gauss-Bessel STED Microscopy

Wentao Yu, Ziheng Ji, Xusan Yang, Yunfeng Xiao, Peng Xi, and Kebin Shi

CLEO: Science and Innovations 2016

San Jose, California United States
5–10 June 2016
ISBN: 978-1-943580-11-8

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Imaging II: Deep Tissue and Super-Resolution (STh4G)

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W. Yu, Z. Ji, X. Yang, Y. Xiao, P. Xi, and K. Shi, "Super-resolution Deep Imaging with Gauss-Bessel STED Microscopy," in Conference on Lasers and Electro-Optics, OSA Technical Digest (2016) (Optica Publishing Group, 2016), paper STh4G.4.

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Abstract

We report a Gauss-Bessel STED microscope for super-resolution deep imaging. Improved lateral resolution at 95μm-depth in agarose phantom of brain tissue is about 110nm, which is nearly 2-fold improvement on resolution comparing to conventional STED imaging.

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