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High-speed Live-cell Super-resolution Microscopy with Stochastically Switching Fluorophores

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Abstract

Super-resolution microscopy with stochastically blinking fluorophores (FPALM/PALM/STORM, etc.) achieves ~25 nm resolution by localizing molecules in thousands of camera frames. Here I present a solution to speed up recording dramatically, enabling nanoscopy at video rate.

© 2013 Optical Society of America

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