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  • 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper cl_5_5

Label-free multiphoton microscopy in human tissue enabled by an Er:fiber-laser based tunable source

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Abstract

Multiphoton microscopy (MPM) is an important bio-imaging tool. Different modalities can serve as a contrast agent, such as second-/third-harmonic generation (SHG/THG) and two-/three-photon excitation fluorescence (2PEF/3PEF). Ultrafast lasers with flexible wavelength tunability are crucial for driving MPM bio-imaging, and the conventional solution relies on ultrafast Ti:sapphire lasers plus an optical parametric oscillator/amplifier. Recently, we have demonstrated that ultrafast fiber lasers are a potential solution to implementing compact, robust, and wavelength tunable femtosecond sources for driving MPM. To realize wavelength tunability we employ self-phase modulation (SPM) in optical fibers to broaden a narrowband input spectrum of Yb-/Er-doped fiber lasers (YDFLs/EDFLs) up to >400-nm wide with well-isolated spectral lobes; filtering the leftmost/rightmost lobes leads to nearly transform-limited pulses [1–6]. Such a SPM-enabled spectral selection (SESS) allows us to obtain wavelength widely tunable femtosecond pulses for MPM [2,5,6].

© 2019 IEEE

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