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  • 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper cl_3_3

A New Pulse-Shaped Multiphoton Single-Molecule Microscope

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Abstract

Single-molecule (SM) fluorescence microscopy can reveal information that is otherwise hidden in the average signal produced by conventional ensemble methods. We are interested in studying the structure and dynamics of DNA, which normally involves attaching a bright extrinsic fluorescent probe [1]. Although there would be benefits to using intrinsic probes such as fluorescent nucleobases, photobleaching, UV absorption and background has so far prevented their practical detection at the single-molecule level.

© 2019 IEEE

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