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  • 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference
  • (Optica Publishing Group, 2015),
  • paper CL_P_12

Probing the structure of collagen in tissues: Forward versus Backward Polarization-resolved SHG microscopy

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Abstract

In situ visualization of fibrillar collagen in biological tissues, such as tendon, skin, bones or cornea, is crucial. This is a major biomedical concern, whether to study collagen accumulation, impairment or disorder in numerous pathologies, or to understand structure of organs and guide tissue engineering. To this goal, second harmonic generation (SHG) is a powerful technique to observe fibrillar collagen, as well as other biopolymers, without any staining and with a good contrast. This is not possible with classic imaging modalities. More information about structure of collagen fibrils in tissues and its changes can be gained with polarization-resolved second-harmonic generation (P-SHG) microscopy [1]. Two quantitative parameters can be measured and are linked with structural information: in-plane orientation of fibrils in the field of view and second harmonic signal anisotropy, which is related to collagen’s molecular structure and 3D orientation inside the excitation volume (submicroscopic scale).

© 2015 IEEE

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