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Conferences > CLEO_Europe > 2015 > CL_2 > CL_2_1
  • 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference
  • (Optica Publishing Group, 2015),
  • paper CL_2_1

Nanophotonic measurement of the dark fraction in red fluorescent proteins

J.C. Prangsma, R. Molenaar, V. Subramaniam, and C. Blum

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Abstract

All complex fluorophores such as fluorescent proteins display a fraction of the population that absorb light but do not emit fluorescence. Accurate measurement of this ‘dark’ fraction is of critical importance for quantitative microscopy in cell biology. We used a nanophotonic method to measure the dark fraction of several frequently used red fluorescent proteins.

© 2015 IEEE

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