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  • CLEO/Europe and EQEC 2009 Conference Digest
  • (Optica Publishing Group, 2009),
  • paper CM_P3

Deposition of pepsin thin layer using IR-MAPLE procedure

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Abstract

Nowadays significant interest turned towards the thin layers of large organic molecules due to their applicability in medicine and biosensor fabrication. Matrix assisted pulsed laser evaporation (MAPLE) is a promising technique for thin layer preparation in the cases of sensitive biomaterials [1,2]. An obviously natural, inert matrix material would be the water, i.e. ice for solid target [3]. However its absorption coefficient in wavelength range of the mostly used, ultraviolet lasers is relative low, a few 1/m [4]. Therefore in our experiments beam of a CO2 laser was used to ablate an enzyme solved in water showing strong absorptivity in far-IR. Our main aim was to demonstrate that the IR-MAPLE is suitable for the deposition of biologically active pepsin thin layer from frozen pepsin-water matrix solution.

© 2009 IEEE

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