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Visualizing Mammalian Brain Area Interactions by Dual-axis Two-photon Calcium Imaging

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Abstract

Fluorescence Ca2+ imaging enables large-scale recordings of neural activity, but collective dynamics across mammalian brain regions are generally inaccessible within single fields of view. Here we introduce a two-photon microscope possessing two articulated arms that can simultaneously image two brain areas (~0.38 mm2 each), either nearby or distal, using microendoscopes, in awake behaving rodents.

© 2015 Optical Society of America

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