Improved spectral resolution in confocal microscopy is important in both materials research and biological studies. When studying dynamic fluorescent samples, it is ideal to image at high spectral resolution while retaining good viability and signal sensitivity, as well as high temporal fidelity. We present a high-speed spectral confocal microscope that utilizes a low-loss Amici prism for spectral separation in conjunction with a multi-point confocal design for high-speed image capture. Our system achieves 15 channels of spectral resolution at a 2 frames per second imaging rate and with minimal loss of overall signal.

© 2013 Optical Society of America

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