Abstract

As a recently developed imaging technology, optical coherence tomography (OCT) has been widely used in medicine, biology as well as material science to acquire cross-sectional tomographic images with a high resolution at micrometer level. However, as a backreflected imaging method, the specificity of OCT for different subject structures is relatively low due to its speckle and other issues. On the other hand, the two-photon microscopy has great specificity especially for collagen and fibrin. This study aims at combining the micro OCT system and 2-photon microscopy in order to enhance OCT’s specificity, and provide related analysis. We are building a combined system consists of these two imaging method, and managed to achieve a precise pixel-to-pixel match of the output of the two imaging systems.

© 2017 Optical Society of America