High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

doi:10.1364/CLEO.2010.CThCC4

Conference on Lasers and Electro-Optics 2010
OSA Technical Digest (CD) (Optica Publishing Group, 2010),
paper CThCC4
•https://doi.org/10.1364/CLEO.2010.CThCC4

High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa

Conference on Lasers and Electro-Optics 2010

San Jose, California United States
16–21 May 2010
ISBN: 978-1-55752-889-6

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Superresolution Imaging (CThCC)

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K. Isobe, A. Suda, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, "High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation," in Conference on Lasers and Electro-Optics 2010, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper CThCC4.

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Abstract

We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore.

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