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Optica Publishing Group
  • Conference on Lasers and Electro-Optics/International Quantum Electronics Conference
  • OSA Technical Digest (CD) (Optica Publishing Group, 2009),
  • paper CTuAA7
  • https://doi.org/10.1364/CLEO.2009.CTuAA7

3D fluorescent particle tracking with nanometer scale accuracies using a double-helix point spread function

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Abstract

We demonstrate parallel three-dimensional (3D) tracking of multiple fluorescent microspheres with nanometer scale accuracies by engineering the 3D point spread function of a wide-field microscope to present a double-helix along the optical axis.

© 2009 Optical Society of America

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