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Conferences > CLEO > 2008 > CFT > CFT5
  • Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference and Photonic Applications Systems Technologies
  • OSA Technical Digest (CD) (Optica Publishing Group, 2008),
  • paper CFT5

FRET detection in the plasma membrane using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy

P. Blandin, S. Lévêque-Fort, S. Lécart, F. Druon, P. Georges, J.C. Cossec, M.C. Potier, and Z. Lenkei

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Abstract

We developed a Total Internal Reflection Fluorescence Lifetime Imaging Microscope to perform functional imaging of living cells membranes labeled with FRET couples. Förster Resonance Energy Transfer efficiency can thus be followed with subwavelength axial resolution.

© 2008 Optical Society of America

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