Abstract
Development of ultraviolet (UV) tunable laser excitation sources permits two-dimensional spectral analysis of fluorescent spectra acquired as a function of excitation wavelength, usually referred to as an excitation-emission matrix (EEM). Recent research on B. subtilus spores indicates that there may be three main component chromophores that contribute significantly to fluorescent emission: tryptophan,1 NADH,2 and other coenzymes, principally riboflavin3 listed in order of decreasing emission strength and increasing emission wavelength. Similar techniques for using spectral fingerprint analysis in a multidimensional data set have also been applied to population characterization for cell sorting using flow cytometry.4 The potential for in situ, optical spectrometric classification of these and other bacteria, based on EEM data, has provided motivation for this research effort.
© 1996 Optical Society of America
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