Abstract
Frequency-domain determination of fluorescence lifetimes has recently been utilized to determine concentrations of biologically important gases, including O2 and CO2 1 This technique measures the change in fluorescence lifetime due to the analyte gas by measuring the phase shift between an intensity-modulated excitation and the modulated emission. By using fluorescence lifetimes instead of intensities, interferences due to probe leaching or bleaching and source-intensity variations due to scattering, absorbance, or other causes can be avoided.
© 1995 Optical Society of America
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