Abstract

Spectroscopic methods have been explored to study pathology in human tissue.¹ Deconvolution of spectra to yield concentrations of tissue components has proven difficult for two reasons: (i) human tissue is a scattering and absorbing medium, and (ii) the spectra of many biological chromophores overlap. Although we have presented preliminary data comparing a number of methods for predicting fluorophore concentration,² a comprehensive study, including samples that exhibit a wide range of optical properties typical of a variety of tissues, has not yet been presented.

© 1995 Optical Society of America