Abstract

The Tabor and Richardson strategy for enzymatic chain termination sequencing of DNA using relative peak intensity has been adapted to capillary gel electrophoresis with laser-induced fluorescence detection (CGE/LIF). This approach to DNA sequencing involves the use of only a single fluor and results in significant reduction in the time required to determine a DNA sequence without the use of highly complicated and expensive instrumentation.

© 1992 Optical Society of America