Abstract
Fluorescence contour mapping is a powerful technique for analyzing fluorescence signals from samples which contain a complex mixture of chromophores and thus cannot be described in terms of a single excitation and emission spectrum.1,2 Fluorescence contour maps (Figs. 1 and 2) consist of a 2-D plot with the excitation wavelength on one axis and the emission wavelength on the other. Contour lines connect points of equal fluorescence intensity.
© 1989 Optical Society of America
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