High-fidelity imaging with Bessel-beam light-sheet microscopy for whole-brain structural and functional studies

M. Caroline Müllenbroich; M. Caroline Müllenbroich; Ludovico Silvestri; Lapo Turrini; Antonino P. Di Giovanna; Tommaso Alterini; Ali Gheisari; Leonardo Sacconi; Leonardo Sacconi; Francesco Vanzi; Francesco S. Pavone; Francesco S. Pavone; Francesco S. Pavone

doi:10.1364/BRAIN.2017.BrW4B.2

Optics in the Life Sciences Congress
OSA Technical Digest (online) (Optica Publishing Group, 2017),
paper BrW4B.2
•https://doi.org/10.1364/BRAIN.2017.BrW4B.2

High-fidelity imaging with Bessel-beam light-sheet microscopy for whole-brain structural and functional studies

M. Caroline Müllenbroich, Ludovico Silvestri, Lapo Turrini, Antonino P. Di Giovanna, Tommaso Alterini, Ali Gheisari, Leonardo Sacconi, Francesco Vanzi, and Francesco S. Pavone

Optics and the Brain 2017

San Diego, California United States
2–5 April 2017
ISBN: 978-1-943580-25-5

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Hybrid Methods and Function/Structure (BrW4B)

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M. C. Müllenbroich, L. Silvestri, L. Turrini, A. P. Di Giovanna, T. Alterini, A. Gheisari, L. Sacconi, F. Vanzi, and F. S. Pavone, "High-fidelity imaging with Bessel-beam light-sheet microscopy for whole-brain structural and functional studies," in Optics in the Life Sciences Congress, OSA Technical Digest (online) (Optica Publishing Group, 2017), paper BrW4B.2.

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Abstract

Bessel beam have attractive properties like their propagation invariance and “self-healing” capabilities. Here we present significant advantages of Bessel illumination in structural and functional imaging in light-sheet microscopy compared to conventional Gaussian beam illumination.

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