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Designing large field-of-view two photon microscopy using optical invariant analysis and off-the-shelf components

Jonathan R. Bumstead, Isaac A. Rosen, Andrew W. Kraft, Patrick W. Wright, Daniel Côté, and Joseph P Culver

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Abstract

Conventional two photon microscopy (TPM) is capable of imaging neural dynamics with subcellular resolution, but is limited to a field of view (FOV) <Ø1mm. We present a strategy for extending the FOV to Ø8mm while maintaining cellular resolution.

© 2017 Optical Society of America

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