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Conferences > BRAIN > 2015 > BrW3B > BrW3B.5

In vivo Three-photon Imaging of Brain Activity from Cortical and Subcortical Neurons in Intact Mouse Brain

Dimitre G. Ouzounov, Tianyu Wang, Nicholas G. Horton, Jean C. Cruz Hernández, Danielle Feng, Nozomi Nishimura, and Chris Xu

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Abstract

We demonstrate three-photon microscopy (3PM) at 1300-nm-excitation for imaging neuronal activity through the entire neocortex and as deep as the hippocampal stratum pyramidale (SP) layer of the living adult mouse brain using genetically-encoded calcium indicators.

© 2015 Optical Society of America

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