Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Phase diversity based object estimation in light-sheet fluorescence microscopy

Not Accessible

Your library or personal account may give you access

Abstract

Light-sheet fluorescence microscopy techniques commonly use deconvolution to remove the effect of the illumination beam shape on the image formation, reducing the effects of side lobes and increasing the contrast in three-dimensional imaging. Deconvolution requires knowledge of the optical transfer function of the system and can only be estimated or measured imperfectly. Furthermore, in biological samples the optical transfer function is degraded by the presence of phase aberrations, this implies that any a priori deconvolution applied to the sample will be to some degree incorrect since it does not account for this unknown phase error in the optical transfer function. By combining adaptive optics and computational processing in this microscope, it is shown that by introducing perturbations to the optical transfer function it is possible to estimate the object distribution and remove aberrations.

© 2017 Optical Society of America

PDF Article
More Like This
Sync-free light sheet microscopy based on a 2-D phase mask

Yina Chang, Chenyang Wen, and Shih-Chi Chen
NTu3C.3 Novel Techniques in Microscopy (NTM) 2017

Estimation of Object Spectral Content Using Phase Diversity

Matthew R. Bolcar and James R. Fienup
FWL1 Frontiers in Optics (FiO) 2007

Light sheet-based fluorescence microscopy (LSFM) reduces phototoxic effects and provides new means for the modern life sciences

Francesco Pampaloni, Nari Ansari, Philippe Girard, and Ernst H.K. Stelzer
80860Y European Conference on Biomedical Optics (ECBO) 2011

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved