Multiplexed fluorescence lifetime image with Fourier excitation-emission spectroscopy

Ming Zhao; Leilei Peng

doi:10.1364/BODA.2011.BWC3

Optics in the Life Sciences
OSA Technical Digest (CD) (Optica Publishing Group, 2011),
paper BWC3
•https://doi.org/10.1364/BODA.2011.BWC3

Multiplexed fluorescence lifetime image with Fourier excitation-emission spectroscopy

Ming Zhao and Leilei Peng

Bio-Optics: Design and Application 2011

Monterey, California United States
4–6 April 2011
ISBN: 978-1-55752-909-1

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Spectroscopic Imaging (BWC)

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M. Zhao and L. Peng, "Multiplexed fluorescence lifetime image with Fourier excitation-emission spectroscopy," in Optics in the Life Sciences, OSA Technical Digest (CD) (Optica Publishing Group, 2011), paper BWC3.

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Abstract

We report a Fourier transform lifetime spectroscopy method simultaneously measures fluorescence lifetime and intensity on multiple excitation and emission channels in 46 microseconds. The technique will allow fast imaging study of multicolor Förster resonance energy transfer.

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