Fluorescence lifetime spectroscopy: a new technique for the characterization of polyplexes

Cosimo D’Andrea; Cosimo D’Andrea; Daniele Pezzoli; Chiara Malloggi; Alessia Candeo; Alessandro Volonterio; Andrea Bassi; Paola Taroni; Paola Taroni; Gabriele Candiani; Gabriele Candiani

doi:10.1364/BIOMED.2014.BS3A.3

Biomedical Optics 2014
OSA Technical Digest (online) (Optica Publishing Group, 2014),
paper BS3A.3
•https://doi.org/10.1364/BIOMED.2014.BS3A.3

Fluorescence lifetime spectroscopy: a new technique for the characterization of polyplexes

Cosimo D’Andrea, Daniele Pezzoli, Chiara Malloggi, Alessia Candeo, Alessandro Volonterio, Andrea Bassi, Paola Taroni, and Gabriele Candiani

Biomedical Optics 2014

Miami, Florida United States
26–30 April 2014
ISBN: 978-1-55752-997-8

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Poster Session I (BS3A)

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C. D’Andrea, D. Pezzoli, C. Malloggi, A. Candeo, A. Volonterio, A. Bassi, P. Taroni, and G. Candiani, "Fluorescence lifetime spectroscopy: a new technique for the characterization of polyplexes," in Biomedical Optics 2014, OSA Technical Digest (online) (Optica Publishing Group, 2014), paper BS3A.3.

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Abstract

Herein we demonstrate that fluorescence lifetime measurements of DNA-dye SYBR Green I provide significant information for the physicochemical characterization of polymeric vectors/DNA complexes (polyplexes). The relationships among fluorescence amplitude/lifetime, structure and transfection efficiency of polyplexes is identified and discussed.

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