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Molecular Laminar Optical Tomography of Thick Tissue Engineered Constructs

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Abstract

We report the integration of a robotic bio-fabrication technology with a mesoscopic fluorescence molecular tomography imaging system. We demonstrate the capability of the platform to construct perfused scaffolds with endothelial lining and to image both the fluid flow and fluorescent-labeled living cells at high-frame rates, with high sensitivity and accuracy. Our results demonstrate the capability of cell printing technology to create in vitro tissues with vascular perfusion, and to allow for rapid, non-contact imaging of live tissue up to a few millimeters in depth.

© 2012 Optical Society of America

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