The dynamic change of NADH fluorescence lifetime in PARP-1 induced cell death

Han-Wen Guo; Yau-Huei Wei; Hsing-Wen Wang

doi:10.1364/BIOMED.2010.BTuD7

Biomedical Optics and 3-D Imaging
OSA Technical Digest (CD) (Optica Publishing Group, 2010),
paper BTuD7
•https://doi.org/10.1364/BIOMED.2010.BTuD7

The dynamic change of NADH fluorescence lifetime in PARP-1 induced cell death

Han-Wen Guo, Yau-Huei Wei, and Hsing-Wen Wang

Biomedical Optics 2010

Miami, Florida United States
11–14 April 2010
ISBN: 978-1-55752-887-2

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H. Guo, Y. Wei, and H. Wang, "The dynamic change of NADH fluorescence lifetime in PARP-1 induced cell death," in Biomedical Optics and 3-D Imaging, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper BTuD7.

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Abstract

We imaged NADH fluorescence lifetime in HeLa cells treated with a PARP-1 activating agent, N-methyl-N′-nitro-N-nitrosoguanidine, and then pytuvate to prevent cell death. NADH fluorescence lifetime may be a potential diagnostic/therapeutic biomarker in PARP-1 induced cell death.

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