Optical Temperature Measurement in Aqueous Nanodroplets for PCR

Hanyoup Kim; Sanhita Dixit; Chia-Pin Pan; Gregory W. Faris

doi:10.1364/BIOMED.2008.BMD69

Biomedical Optics
OSA Technical Digest (CD) (Optica Publishing Group, 2008),
paper BMD69
•https://doi.org/10.1364/BIOMED.2008.BMD69

Optical Temperature Measurement in Aqueous Nanodroplets for PCR

Hanyoup Kim, Sanhita Dixit, Chia-Pin Pan, and Gregory W. Faris

Biomedical Optics 2008

St. Petersburg, Florida United States
16–19 March 2008
ISBN:

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BIOMED Poster Session II (BMD)

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H. Kim, S. Dixit, C. Pan, and G. W. Faris, "Optical Temperature Measurement in Aqueous Nanodroplets for PCR," in Biomedical Optics, OSA Technical Digest (CD) (Optica Publishing Group, 2008), paper BMD69.

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Abstract

We are investigating temperature measurements of nanoliter droplets to support polymerase chain reaction (PCR) assays. A fluorescence quenching method appears most promising for this application.

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