Enzyme activity imaging with confocal fluorescence polarization microscopy

Chad E. Bigelow; John G. Frelinger; Thomas H. Foster

doi:10.1364/BIO.2004.FH14

Biomedical Topical Meeting
OSA Technical Digest (Optica Publishing Group, 2004),
paper FH14
•https://doi.org/10.1364/BIO.2004.FH14

Enzyme activity imaging with confocal fluorescence polarization microscopy

Chad E. Bigelow, John G. Frelinger, and Thomas H. Foster

Biomedical Topical Meeting 2004

Miami Beach, Florida United States
14–17 April 2004
ISBN: 1-55752-769-5

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Poster Session III (FH)

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C. E. Bigelow, J. G. Frelinger, and T. H. Foster, "Enzyme activity imaging with confocal fluorescence polarization microscopy," in Biomedical Topical Meeting, OSA Technical Digest (Optica Publishing Group, 2004), paper FH14.

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Abstract

We have developed a technique for imaging enzyme activity based on confocal fluorescence polarization microscopy. Efficacy of the technique is demonstrated in model systems and experiments are performed to test its limits in scattering systems.

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