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Laminar Optical Tomography of Rat Cortical Activation:resolving depth-dependent hemodynamics from 0 to 2mm.

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Abstract

Laminar Optical Tomography (LOT) is a new technique for imaging rat cortex, combining the advantages of Diffuse Optical Tomography image reconstruction, with a microscopy-based set-up to allow imaging with 100 to 200µm resolution effective over depths of 0 to 2mm. System design and image reconstruction techniques are described, alongside phantom data and in-vivo (thinned skull) rat data identifying activation in response to forepaw stimulation and revealing different activation time-courses corresponding to different depths.

© 2004 Optical Society of America

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