Fluorescence lifetime imaging of DNA microarrays for expression profiling

Daniela Comelli; Cosimo D’Andrea; Gianluca Valentini; Rinaldo Cubeddu; Clarissa Consolandi; Gianluca De Bellis; Luigi Rossi-Bernardi

doi:10.1364/BIO.2002.WD2

Biomedical Topical Meeting
(Optica Publishing Group, 2002),
paper WD2
•https://doi.org/10.1364/BIO.2002.WD2

Fluorescence lifetime imaging of DNA microarrays for expression profiling

Daniela Comelli, Cosimo D’Andrea, Gianluca Valentini, Rinaldo Cubeddu, Clarissa Consolandi, Gianluca De Bellis, and Luigi Rossi-Bernardi

Biomedical Topical Meeting 2002

Miami Beach, Florida United States
7–10 April 2002
ISBN: 1-55752-702-4

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D. Comelli, C. D’Andrea, G. Valentini, R. Cubeddu, C. Consolandi, G. De Bellis, and L. Rossi-Bernardi, "Fluorescence lifetime imaging of DNA microarrays for expression profiling," in Biomedical Topical Meeting, (Optica Publishing Group, 2002), paper WD2.

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Abstract

Time resolved fluorescence imaging has been applied to DNA-microarray expression profiling. Discrimination between two fluorescent labels is performed on the basis of their lifetimes. Quantification of the relative amount of each marker is inferred from the relative fluorescence amplitude. Measurements on test DNA microarrays reveal that the temporal domain is a valid alternative to the spectral one.

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